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	Provedor de dados BJM (1) Autor Adnan,Ahmad (1) Ahmad,Waqar (1) Shabbiri,Khadija (1) Syed,Quratulain (1) Palavra-chave Complex II (1) Cytochrome b (1) Proteus mirabilis (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Isolation and purification of complex II from proteus mirabilis strain ATCC 29245 BJM Shabbiri,Khadija; Ahmad,Waqar; Syed,Quratulain; Adnan,Ahmad. A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate:... Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteus mirabilis; Complex II; Cytochrome b. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032 Registros recuperados: 1 Primeira ... 1 ... Última

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